ANTIOXIDANT GLYCOSAMINOGLYCANS EXTRACTED FROM THE SKIN OF "TAMBATINGA", A HYBRID FISH
DOI:
https://doi.org/10.18817/repesca.v16i3.3711Resumo
Market of "tambatinga" (Colossoma macropomum × Piaractus brachypomus) has grown in Brazil, but its filleting residue glycosaminoglycans (TbGAGs) are unexplored. This study aimed at analyzing TbGAGs, extracted from skin, on their structural and antioxidant properties. Quantified fresh skin was dehydrated for extraction with papain, in 100mM sodium acetate buffer (pH5)+5mM cysteine/EDTA. Physical-chemical features of TbGAGs were examined by electrophoreses (agarose/polyacrylamide gels) vs. standard GAGs stained with toluidine blue or Stains-All; and then structurally by infrared technique. TbGAGs were in vitro tested, for antioxidant effects, on the DPPH, total antioxidant capacity (TAC) and ferrous ion chelating assays vs. BHT, ascorbic acid and EDTA, respectively. Results of fresh skin (~3.95% fish-1) and TbGAGs extraction (0.37±0.03%) presumed a dermatan (~40kDa) with structural complexity from the molecular analyses. Regarding assays, TbGAGs were concentratio-dependent antioxidants, but there was preponderance on the TAC, although less effective than respective synthetics. Thus, "tambatinga" has skin antioxidant GAGs and analyses from progenitors suggest future studies.