BIOCHEMICAL PROSPECTION OF GLYCOSAMINOGLYCANS IN FISH WASTES AIMING AT CIRCULAR ECONOMY
DOI:
https://doi.org/10.18817/repesca.v16i3.4116Resumo
Wastes of Prochilodus brevis and Oreochromis niloticus fishes could be reservoirs in glycosaminoglycans (GAGs) for circular economy. The aim of this study was to biomass prospect for biochemical analysis of GAGs, from cultured fish wastes, on yield and physical/chemical/structural features. Dehydrated wastes showed both skins (0.51/0.65%, w w-1, respectively) with higher volume than O. niloticus eyeball (0.29%), intestine (0.08%) and gonad (0.03%) from the total fish mass. Papain digestion had an inverse rate (p < 0.05) in yield (%) of GAGs (0.35 ± 0.03/0.18 ± 0.02-skin; 0.16 ± 0.03-eyeball; 0.36 ± 0.10-intestine; 0.40 ± 0.08-gonad). Combined electrophoresis and infrared techniques revealed a difference in charge density among the samples in mixture or not of GAGs, suggesting dermatan (skin), chondroitin/hyaluronic acid (eyeball), heparan/hyaluronic acid (intestine) and dermatan/hyaluronic acid (gonad) the classes found from ~8 to >100 kDa. The scenario presumed a dominant yield of GAGs in abdominal cavity while a more charged composition for those located in external anatomy. Therefore, the study contributed to partial description of freshwater fish waste GAGs of bioeconomic interest.